Cloned (Comment) | Organism |
---|---|
overexpression of the alpha and beta subunits and the C- and N-terminal parts of the Escherichia coli enzyme in Escherichia coli as monomeric and dimeric mutants, also mixed between the species | Aquifex aeolicus |
overexpression of the N-terminal and the C-terminal parts of the enzyme and of the alpha- and beta-subunits of the Aquifex aeolicus enzyme in an Escherichia coli strain as monomeric and dimeric mutants, also mixed between the species | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the last 36 residues of the alpha-subunit is deleterious for tRNA charging, resulting in an inactive mutant, the monomeric mutants containing the alpha-subunit have activities comparable to the wild-type enzyme, while the heterodimeric enzymes show very low activity | Aquifex aeolicus |
additional information | overexpression of the alpha and beta subunits of the Aquifex aeolicus enzyme and the C- and N-terminal parts of the Escherichia coli enzyme in Escherichia coli as monomeric and dimeric mutants, also mixed between the species, the heterodimeric mutants and the monomeric mutants containing the N-terminal-subpart of the enzyme show very low activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, wild-type and mutant enzymes | Escherichia coli | |
additional information | - |
additional information | kinetics, wild-type and mutant enzymes | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Escherichia coli | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | Aquifex aeolicus | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activiy of several enzyme mutants and mixed mutants, containing subparts of the Escherichia coli enzyme | Escherichia coli |
additional information | - |
activiy of several enzyme mutants and mixed mutants, containing subparts of the Escherichia coli enzyme | Aquifex aeolicus |
0.047 | - |
purified recombinant wild-type enzyme, with substrate tRNALeu from Escherichia coli | Aquifex aeolicus |
0.235 | - |
purified recombinant wild-type enzyme, with substrate tRNALeu from Aquifex aeolicus | Aquifex aeolicus |
1.3 | - |
purified recombinant wild-type enzyme, with substrate tRNALeu from Aquifex aeolicus | Escherichia coli |
1.5 | - |
purified recombinant wild-type enzyme, with substrate tRNALeu from Escherichia coli | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | the cross-species-specific recognition occurs at the alpha-subunit, tRNALeu substrates from Escherichia coli and Aquifex aeolicus | Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | tRNALeu substrates from Escherichia coli and Aquifex aeolicus | Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Leucine translase | - |
Escherichia coli |
Leucine translase | - |
Aquifex aeolicus |
Leucine--tRNA ligase | - |
Escherichia coli |
Leucine--tRNA ligase | - |
Aquifex aeolicus |
Leucyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Leucyl-transfer ribonucleate synthetase | - |
Aquifex aeolicus |
Leucyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Leucyl-transfer ribonucleic acid synthetase | - |
Aquifex aeolicus |
Leucyl-transfer RNA synthetase | - |
Escherichia coli |
Leucyl-transfer RNA synthetase | - |
Aquifex aeolicus |
Leucyl-tRNA synthetase | - |
Escherichia coli |
Leucyl-tRNA synthetase | - |
Aquifex aeolicus |
LeuRS | - |
Escherichia coli |
LeuRS | - |
Aquifex aeolicus |
Synthetase, leucyl-transfer ribonucleate | - |
Escherichia coli |
Synthetase, leucyl-transfer ribonucleate | - |
Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the mixed enzyme mutants are not thermostable in contrary to the wild-type enzyme | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Aquifex aeolicus |